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TALEN™ Hit software : assess your target sequence for potential TALEN™ hits.
Cellectis bioresearch’s TALEN™ design can identify potential hits every couple of nucleotides.
This makes numerous projects that were previously unachievable, now possible.
Please paste in the box below the name and the defined sequence to be targeted in FASTA format.
The defined sequence is understood as followed, according to your genome customization project :
For a gene knock-out project : the defined sequence refers to 200bps contained within an exon located within the first third or half of the genomic sequence to be disrupted.
Note: A functional knock-out is understood as a modification resulting in a frame shift of the coding sequence. This does not require an integration or conversion matrix.
For a nucleotide substitution project1 : the defined sequence is 200bps containing the substitution site, centered within this defined sequence.
For a transgene integration project1 : the defined sequence is 200bps containing the integration site, centered within this defined sequence.
For promoter swap project1 : the defined sequence is 200bps containing the regulatory site, centered within this defined sequence.
For gene tagging project1 : the defined sequence is 200bps containing the N-ter or C-ter site. The start or stop codon, respectively, should be centered within the defined sequence.
1 For any applications requiring an integration or conversion matrix, please note that the integration site should be as near as possible (i.e. within 200bps) of the cleavage site, but does not have to be contained within the cleavage site. The integration or conversion matrix that contains homology arms will allow the integration of the nucleotides to be substituted (nucleotide substitution project) or added (transgene integration, promoter swap and gene tagging projects).
Please note that you must verify that the defined sequence provided is exactly the sequence present in your particular cell line/organism of study, especially if data is coming from a database (i.e. GenBank). Any mismatches between the defined sequence provided and the existing sequence within the genome of your cells of interest can prevent Custom TALEN™ binding and overall efficiencies.
Spaces and digits are tolerated and removed from final FASTA Format.
Regions of interest in your sequence can be highlighted using CAPITAL letters (will be present in the results).
Warnings :
- Maximum Sequence Length : 250 bps.
- Maximum number of sequences by request : 50.
- if you want to paste sequences from NCBI, display them as Text first.
- In FASTA format space characters in sequence headers will be replaced by underscore characters.